详细说明
- Species ReactivityHuman, Mouse
- SpecificityDetects human and mouse BRCA1 C-Terminus.
- SourceMonoclonal Mouse IgG 2B Clone # 440621
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenE. coli-derived recombinant human BRCA1 C-Terminus
Arg1634-Tyr1863
Accession # P38398 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
Applications
- Recommended
ConcentrationSample
- Western Blot1 µg/mLSee below
- Immunohistochemistry8-25 µg/mLSee below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
| Immunohistochemistry | BRCA1 in Human Breast Cancer Tissue. BRCA1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human BRCA1 Monoclonal Antibody (Catalog # MAB22101) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # ). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ) and counterstained with hematoxylin (blue). View our protocol for . |
| Immunohistochemistry | BRCA1 in Human Breast. BRCA1 was detected in immersion fixed paraffin-embedded sections of human breast array using Human BRCA1 Monoclonal Antibody (Catalog # MAB22101) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for . |
| Western Blot | Detection of Human/Mouse BRCA1 by Western Blot. Western blot shows lysates of CH‑1 mouse B cell lymphoma cell line, BaF3 mouse pro-B cell line, U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human BRCA1 Monoclonal Antibody (Catalog # MAB22101) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for BRCA1 at approximately 240 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Preparation and Storage
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BRCA1
The BRCA1 ( BReast CAncer gene 1) tumor suppressor protein has many reported functions. In addition to mediating signal transduction in DNA damage and repair responses, BRCA1 forms a heterodimer with BARD1 and regulates transcriptional activity, assisting in the preservation of chromosomal stability. BRCA1 is one of the first proteins recruited to sites of DNA double-strand breaks and serves as part of a scaffold for assembling other DNA damage response or repair factors, including MSH2, MLH1, ATM and PMS2.
- Long Name:Breast Cancer 1
- Entrez Gene IDs:672 (Human); 12189 (Mouse); 497672 (Rat)
- Alternate Names:BRCA1; BRCAI; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein; breast cancer 1, early onset; breast cancer type 1 susceptibility protein; EC 6.3.2; EC 6.3.2.-; IRIS; PNCA4; PSCP; subunit 1
400-6226-992