详细说明
- Species ReactivityHuman
- SpecificityDetects human Neprilysin/CD10 in Western blots and direct ELISAs. In Western blots, less than 5% cross-reactivity with recombinant human ECE-2 and recombinant mouse Neprilysin is observed.
- SourceMonoclonal Mouse IgG2b Clone # 212504
- ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human Neprilysin/CD10
Met45-Pro743
Accession # P08473 - FormulationSupplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
- LabelAlexa Fluor 405
Applications
- Recommended
ConcentrationSample
- Flow Cytometry0.25-1 µg/10 6 cellsDaudi human Burkitt's lymphoma cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Neprilysin/CD10
Neprilysin (NEP) is also known as CALLA and CD10. It is a zinc metalloprotease expressed at the cell surface that cleaves a variety of bioactive peptides such as enkephalins, atrial natriuretic peptide and amyloid beta peptide.
- Entrez Gene IDs:4311 (Human); 17380 (Mouse); 24590 (Rat)
- Alternate Names:Atriopeptidase; CALLA; CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); CD10 antigen; CD10; CD10); CD10membrane metallo-endopeptidase variant 1; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24; EC 3.4.24.11; Enkephalinase; EPN; Leu-19; membrane metallo-endopeptidase variant 2; membrane metallo-endopeptidase; MGC126681; MGC126707; MME; NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA; Neprilysin; Neutral Endopeptidase 24.11; Neutral endopeptidase; NKH1; SFE; Skin fibroblast elastase
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