• Species Reactivity

  Human

• Specificity

  Detects human RELT/TNFRSF19L in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) 4-1BB, recombinant mouse (rm) 4-1BB, rhBAFF R, rmBAFF R, rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rmDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFas, rrFas, rhGITR, rmGITR, rhHVEM, rhLT beta  R, rmLT beta  R, rhNGF R, rmNGF R, rhOPG, rmOPG, rmOX40, rhRANK, rmRANK, rhTNF RI, rmTNF RI, rhTNF RII, rmTNF RII, rhTRAIL R1, rhTRAIL R2, rhTRAIL R3, rhTRAIL R4, rhTROY, or rmTROY is observed.

• Source

  Monoclonal Mouse IgG2b Clone # 238104

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human RELT/TNFRSF19L    
  Met1-Ala160 (Arg127Gly, Arg129Gly)    
  Accession # Q969Z4

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 700

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Raji human Burkitt's lymphoma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: RELT/TNFRSF19L

RELT (Receptor Expressed in Lymphoid Tissues) is a type I transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF19-like (TNFRSF19L) (1, 2). It is primarily expressed in hematopoietic tissues and peripheral blood leukocytes. Human RELT cDNA encodes a 430 amino acid (aa) residue precursor protein with a putative 26 aa signal peptide, a 136 aa extracellular domain containing one TNF receptor cysteine-rich domain and one potential N-linked glycosylation site, a 21 aa transmembrane domain and a 247 aa cytoplasmic region containing no death domain. Human RELT shares 85% and 96% aa sequence homology with mouse RELT (Accession # BAC40459) and macaque RELT (Accession # Q9N092), respectively. Among TNFRSF members, the RELT extracellular domain is most closely related to that of TNFRSF19 and OX40. RELT has been shown to exclusively bind the adaptor protein TNF receptor-associated factor 1 (TRAF1). However, it has also been shown to activate the NF-kappa B pathway independently of TRAFs. Immobilized RELT can co‑stimulate T‑cell proliferation in the presence of CD3 signaling, suggesting a potential regulatory role in immune response.

• References:

1. .

2. Sica, G. et al. (2001) Blood 97:2702.

• Long Name:

  Receptor Expressed in Lymphoid Tissues

• Entrez Gene IDs:

  84957 (Human); 320100 (Mouse)

• Alternate Names:

  Receptor expressed in lymphoid tissues; RELT tumor necrosis factor receptor; RELT; TNFRSF19L; TNFRSF19LFLJ14993; tumor necrosis factor receptor superfamily member 19L; tumor necrosis factor receptor superfamily, member 19-like