详细说明
- Species ReactivitySheep
- SpecificitySheep IgG specific IgG was purified by sheep IgG (H+L) affinity chromatography and absorbed against human IgG, and human, mouse, bovine, and rabbit serum to eliminate cross-reactivity.
- SourcePolyclonal Donkey IgG
- PurificationAntigen Affinity-purified
- ImmunogenSheep IgG
- FormulationSupplied in a saline solution containing BSA and Sodium Azide.
- LabelAllophycocyanin
Applications
- Recommended
ConcentrationSample
- Flow CytometryDesigned for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of sheep origin, and is of IgG class. The recommended concentration is 10 μL/10 6 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
| Flow Cytometry | Detection of SheepIgG Primary Antibody by Flow Cytometry Human peripheral blood granulocytes were stained with SheepAnti-Human Fc epsilon RI alpha Antigen Affinity-purified Polyclonal Antibody (Catalog #,filled histogram) or isotype control antibody (Catalog # ),open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgGSecondary Antibody (Catalog # F0127) at 10 μL/106 cells. |
Preparation and Storage
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageProtect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
- Long Name:Immunoglobulin G
- Alternate Names:IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
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