详细说明
Species Reactivity
Mouse
Specificity
Detects endogenous mouse c-Rel in Western blots.
Source
Monoclonal Rat IgG 2A Clone # 290512
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse c-Rel
Met1-Ile588
Accession # P15307Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
0.5 µg/mL
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Western Blot | Detection of Mouse c-Rel by Western Blot. Western blot shows lysates of DA3 mouse myeloma cell line, A20 mouse B cell lymphoma cell line, and CH‑1 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse c‑Rel Monoclonal Antibody (Catalog # MAB2699) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # ). A specific band was detected for c-Rel at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: c-Rel
c-Rel is a member of the NF kappa B/Rel family of transcription factors. NF kappa B/Rel dimers containing c-Rel regulate expression of genes involved in lymphoid cell development, proliferation, and survival.
Long Name:
Cellular Repressor of E1A-stimulated Genes
Entrez Gene IDs:
5966 (Human); 19696 (Mouse)
Alternate Names:
C-Rel proto-oncogene protein; cRel; c-Rel; I-Rel; oncogene REL, avian reticuloendotheliosis; proto-oncogene c-Rel; REL; v-rel avian reticuloendotheliosis viral oncogene homolog; v-rel reticuloendotheliosis viral oncogene homolog (avian)