• Species Reactivity

  Human, Mouse

• Specificity

  Detects mouse AMIGO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human (rh) AMIGO2 and no cross-reactivity with rhAMIGO1 or recombinant mouse AMIGO3 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 290728

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse AMIGO2    
  Met39-Ala391    
  Accession # Q80ZD9

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human and mouse AMIGO2

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMIGO2

AMIGO2 is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine-rich repeats (LRRs) and one Ig domain in the extracellular region. AMIGO2 is expressed primarily in cerebellum, retina, liver, and lung. AMIGO family proteins are cell adhesion molecules that exhibit homophilic and heterophilic binding properties.

• Long Name:

  Amphoterin-induced Gene and ORF 2

• Entrez Gene IDs:

  347902 (Human); 105827 (Mouse)

• Alternate Names:

  adhesion molecule with Ig-like domain 2; ALI1amphoterin induced gene 2; alivin 1; Alivin-1; AMIGO2; AMIGO-2; DEGAamphoterin-induced protein 2; differentially expressed in gastric adenocarcinoma; Differentially expressed in gastric adenocarcinomas; transmembrane protein AMIGO2