• Species Reactivity

  Mouse

• Specificity

  Detects mouse Frizzled-9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) Frizzled-1, -2, -3, -4, -6, -7, -8, rmFRP, or recombinant human Frizzled-5 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 291004

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant mouse Frizzled‑9    
  Ala23-Asp186 (predicted)    
  Accession # Q9R216

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Mouse Frizzled‑9 under non-reducing conditions only

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-9

Frizzled-9 belongs to the Frizzled family of seven-transmembrane receptors, which are signaling receptors for the family of secreted Wnt ligands. The amino-terminal extracellular cysteine-rich domains of human and mouse Frizzled-9 share 94% amino acid sequence homology.

• Entrez Gene IDs:

  8326 (Human); 14371 (Mouse)

• Alternate Names:

  CD349 antigen; CD349; frizzled (Drosophila) homolog 9; frizzled homolog 9 (Drosophila); Frizzled9; Frizzled-9; fz-9; FZD3fzE6; FZD9; FzE6; hFz9