• Species Reactivity

  Human

• Specificity

  Detects human BAI1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human BAI3 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 542515

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human BAI1    
  Met1-Thr879    
  Accession # O14514

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunohistochemistry

  8-25 µg/mL

  Immersion fixed paraffin-embedded sections of human brain (cerebellum)

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAI1

Brain Angiogenesis Inhibitor 1 (BAI1) is a 170 kDa 7-transmembrane domain G protein-coupled receptor (GPCR) that has a large N-terminal extracellular region with an RGD motif, five thrombospondin type I repeats, and a juxtamembrane GPS (GPCR proteolytic cleavage site) (1). Within the extracellular domain (ECD) up to the GPS (amino acids 31‑879), mature human BAI1 shares 94% amino acid sequence identity with mouse and rat BAI1. BAI1 is preferentially expressed on brain neurons but also is found on astrocytes and macrophages and in the pancreas, stomach, and colon (1‑8). BAI1 can be cleaved within the GPS to release a 120 kDa fragment termed Vasculostatin which corresponds to nearly the entire N-terminal ECD (9). Generation of additional soluble fragments suggests the cleavage of BAI1 at multiple sites (9, 10). BAI1 fragments interact with Integrin alpha V beta 5 or CD36 on microvascular endothelial cells to inhibit cell proliferation and migration (10, 11). Overexpression of BAI1 in glioblastoma or pancreatic adenocarcinoma cells inhibits their tumorigenicity and the development of tumor-associated neovascularization (6, 12). Fragments of the ECD, including Vasculostatin, also suppress   in vivo angiogenesis and tumor growth (1, 9, 11). BAI1 is down‑regulated in glioblastoma, carcinomas of the pancreas, colon, and stomach and also in experimental ischemia (2, 4, 6‑8). Its expression is inversely correlated with tumor vascularity in colorectal and pulmonary carcinomas (8, 13). On macrophages and astrocytes, BAI1 mediates the phagocytosis of apoptotic cells through recognition of cell surface phosphatidylserine (5).

• References:

1. Nishimori, H. et al. (1997) Oncogene 15:2145.

2. Koh, J.T. et al. (2001) Brain Res. Mol. Brain Res. 87:223.

3. Mori, K. et al. (2002) Neurosci. Res. 43:69.

4. Kaur, B. et al. (2003) Am. J. Pathol. 162:19.

5. Park, D. et al. (2007) Nature 450:430.

6. Duda, D.G. et al. (2002) Br. J. Cancer 86:490.

7. Lee, J.H. et al. (2001) Int. J. Oncol. 18:355.

8. Fukushima, Y. et al. (1998) Int. J. Oncol. 13:967.

9. Kaur, B. et al. (2005) Oncogene 24:3632.

10. Koh, J.T. et al. (2004) Exp. Cell Res. 294:172.

11. Kaur, B. et al. (2009) Cancer Res. 69:1212.

12. Kang, X. et al. (2006) Cancer Gene Ther. 13:385.

13. Hatanaka, H. et al. (2000) Int. J. Mol. Med. 5:181.

• Long Name:

  Brain-specific Angiogenesis Inhibitor 1

• Entrez Gene IDs:

  575 (Human); 107831 (Mouse); 362931 (Rat)

• Alternate Names:

  BAI1; brain-specific angiogenesis inhibitor 1; FLJ41988; GDAIF