• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human, mouse and rat Thioredoxin-2 in Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 543317

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Thioredoxin-2    
  Thr60-Gly166    
  Accession # Q99757

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.5 µg/mL

  See below

• 
  

• Simple Western

  5 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thioredoxin-2

Thioredoxin-2 (Trx-2; also known as mitochondrial Thioredoxin) belongs to the evolutionarily conserved Thioredoxin family of proteins. Thioredoxins share the Thioredoxin fold containing the active site-CGPC motif. In their reduced form, the active site cysteine residues reduce protein disulfides. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by a NADPH-dependent Thioredoxin reductase. Thioredoxin-2 contains an N-terminal 59 amino acid (aa) transit sequence that is cleaved upon translocation to mitochondria. The amino acid sequence of mature human Thioredoxin-2 is 98% identical to mouse and rat Thioredoxin-2. Thioredoxin-2 interacts with specific components of the mitochondrial respiratory chain and helps regulate the membrane potential. Thioredoxin-2 is ubiquitously but variably expressed and high expression confers resistance to oxidant-induced apoptosis.

• Entrez Gene IDs:

  25828 (Human); 56551 (Mouse); 79462 (Rat)

• Alternate Names:

  mitochondrial thioredoxin; MTRX; MT-TRX; thioredoxin 2; thioredoxin, mitochondrial; Thioredoxin2; Thioredoxin-2; Trx2; TXN2