• Species Reactivity

  Human, Mouse

• Specificity

  Detects human and mouse MSK2 in Western blots. Does not detect recombinant human MSK1.

• Source

  Monoclonal Rat IgG    _2A Clone # 261034

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human MSK2    
  Met1-Ser772    
  Accession # O75676

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSK2

Mitogen- and stress-activated protein kinase 2 (MSK2), also known as ribosomal protein S6 kinase B (RSKB) and RPS6KA4, belongs to the family of AGC kinases. Both MSK2 and the related MSK1 have two kinase domains connected by a regulatory linker region and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.

• Long Name:

  Mitogen- and Stress-activated Protein Kinase 2

• Entrez Gene IDs:

  8986 (Human); 56613 (Mouse)

• Alternate Names:

  MSK2; ribosomal protein S6 kinase, 90kDa, polypeptide 4; RPS6KA4; RSKB