• Species Reactivity

  Mouse

• Specificity

  Detects mouse DcTRAIL R2/TNFRSF22 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) 4-1BB, rmBAFF R, rmCD27, rmCD30, rmCD40, rmDcTRAIL R1, rmDR3, rmEDAR, rmFas, rmGITR, rmHVEM, rmLT beta  R, rmNGF R, rmOPG, rmOX40, rmRANK, rmTNF RI, and rmTNF RII is observed.

• Source

  Monoclonal Rat IgG    _2B Clone # 263310

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse DcTRAIL R2/TNFRSF22    
  Ala39-Arg175    
  Accession # Q9ER62

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Mouse DcTRAIL R2/TNFRSF22 (Catalog # )

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DcTRAIL R2/TNFRSF22

TRAIL interacts via a complex receptor system to preferentially induce apoptosis of tumor cells in vivo and in vitro (1). In human, two signaling receptors (TRAIL R1 and TRAIL R2) and three decoy receptors (TRAIL R3, TRAIL R4, and OPG) for TRAIL exist. Mouse homologues of human TRAIL R1, -3, and -4 have not been found. Three novel mouse genes (TNF RH1, -2, and -3) that encode proteins with the classic TNF receptor cysteine-rich domains (CRDs) have been identified. These mouse genes are closely clustered on distal mouse chromosome 7 and do not have orthologues in the corresponding human chromosomal region (2). TNF RH1 and -2 have been implicated as decoy receptors for mouse TRAIL and named mDcTRAIL R1/TNFRSF23 and mDcTRAIL R2/TNFRSF22, respectively. The ligand for TNF RH3 is not known (1). The mouse decoy TRAIL R2 (mDcTRAIL R2) gene encodes a 198 amino acid (aa) type I transmembrane protein with a putative 39 aa signal peptide, a 136 aa extracellular domain, a 18 aa transmembrane region, and a 5 aa cytoplasmic tail (1, 2). An alternatively spliced variant encoding a secreted soluble receptor of 180 aa has also been described. The transmembrane and soluble receptors are known as mDcTRAIL R2_L and mDcTRAIL R2_S, respectively. Both mDcTRAIL R2 isoforms and the GPI-linked mDcTRAIL R1 bind TRAIL specifically but are incapable of triggering signal transduction. Mouse DcTRAIL R1 and mDcTRAIL R2 share 71% sequence identity (3). The two receptors have similar structural modules in their CRDs, which differ from those found in the CRDs of mTNF RH3 (2, 3). Mouse DcTRAIL R1 preferentially binds mTRAIL while mDcTRAIL R2 binds both human and mTRAIL. mRNA of mouse DcTRAIL R1 and -2 are expressed at low levels in many mouse tissues and at higher levels in several mouse cell lines.

• References:

1. Engemann, S. et al. (2000) Hum. Mol. Genet. 9:2691.

2. Schneider, P. et al. (2003) J. Biol. Chem. 278:5444.

3. Bodmer, J.-L. et al. (2002) Trends Biochem. Sci. 27:19.

• Long Name:

  Decoy TRAIL Receptor 2

• Entrez Gene IDs:

  79202 (Mouse)

• Alternate Names:

  DcTRAIL R2; DcTRAILR2; SOBa; TNFRH2; TNFRSF22