详细说明
- Species ReactivityMouse
- SpecificityDetects mouse EphB3 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) EphB3 is observed and no cross-reactivity with recombinant rat EphB1, recombinant mouse (rm) EphB4, rmEphB2, rmEphB7, rmEphA4, rmEphA3, rhEphB2, or rhEphA5 is observed.
- SourceMonoclonal Rat IgG 2A Clone # 521002
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenMouse myeloma cell line NS0-derived recombinant mouse EphB3
Leu30-Leu554
Accession # P54754 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Recommended
ConcentrationSample
- Western Blot2 µg/mLSee below
Western Blot | Detection of Mouse EphB3 by Western Blot. Western blot shows lysates of mouse embryo (E19) tissue under reducing (R) and non-reducing (NR) conditions. PVDF membrane was probed with 2 µg/mL Rat Anti-Mouse EphB3 Monoclonal Antibody (Catalog # MAB432) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # ). A specific band for EphB3 was detected at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- References:
- Pasquale, E.B. (2008) Cell 133:38.
- Ruiz, J.C. et al. (1994) Mech. Dev. 48:153.
- Pasquale, E.B (2004) Nat. Neurosci. 7:417.
- Trivier, E. and T.S. Ganesan (2002) J. Biol. Chem. 277:23037.
- Miao, H. et al. (2005) J. Biol. Chem. 280:923.
- Adams, R.H. et al. (1999) Genes Dev. 13:295.
- Krull, C.E. et al. (1997) Curr. Biol. 7:571.
- Willson, C.A. et al. (2006) J. Mol. Histol. 37:369.
- Cortina, C. et al. (2007) Nature Genet. 39:1376.
- Birgbauer, E. et al. (2000) Development 127:1231.
- Alfaro, D. et al. (2008) Immunology 125:131.
- Risley, M. et al. (2009) Mech. Dev. 126:230.
- Liu, X. et al. (2006) J. Neurosci. 26:3087.
- Batlle, E. et al. (2005) Nature 435:1126.
- Holmberg, J. et al. (2006) Cell 125:1151.
- Long Name:Eph Receptor B3
- Entrez Gene IDs:2049 (Human); 13845 (Mouse)
- Alternate Names:Cek10; EC 2.7.10; EC 2.7.10.1; EK2; EPH receptor B3; EphB3; EPH-like kinase 2; EPH-like tyrosine kinase-2; ephrin type-B receptor 3; ETK2Hek2; Hek2; human embryo kinase 2; Mdk5; Sek4; Tyro6; Tyrosine-protein kinase TYRO6