• Species Reactivity

  Mouse

• Specificity

  Detects mouse IL-23 in flow cytometry.

• Source

  Monoclonal Rat IgG    _2A Clone # 320244

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse IL-23 p19 and recombinant mouse IL-12/IL-23 p40    
  Leu20-Ala196 (Il-23 p19) & Met23-Ser335 (IL-12/IL-23 p40)    
  Accession # Q9EQ14 (Il-23 p19) & P43432 (IL-12/IL-23 p40)

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-23

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNA encodes a 196 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent pro-inflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8^- and CD8⁺ subtypes, however only IL-23 can act directly on CD8⁺ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

• References:

1. Oppmann, B. et al. (2000) Immunity 13:715.

2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.

3. Parham, C. et al. (2002) J. Immunol. 168:5699.

4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.

5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

• Long Name:

  Interleukin 23

• Entrez Gene IDs:

  51561 (Human); 83430 (Mouse)

• Alternate Names:

  IL23; IL-23; IL23A; IL-23A; IL-23-A; IL-23p19; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha