• Species Reactivity

  Human

• Specificity

  Detects human ROR alpha /NR1F1 in direct ELISAs.    
   

• Source

  Monoclonal Mouse IgG2b Clone # 784652

• Immunogen

  E. coli-derived recombinant human ROR alpha /NR1F1    
  Arg47-His163    
  Accession # P35398

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 405

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Th17 cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # )

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ROR alpha/NR1F1

RORa (Retinoid-related Orphan Receptor alpha ) is a member of the NR1 nuclear hormone receptor family. ROR alpha is a DNA binding transcription factor, and can bind as a monomer or homodimer to hormone response elements to enhance expression.  Knockout mice implicate ROR alpha as being essential in the development of the cerebellum. It has also been reported to regulate lymphocyte development and play a role in Th17 differentiation. Human ROR alpha shares 99% amino sequence identity with mouse ROR alpha.

• Long Name:

  Retinoic Acid-Related Orphan Receptor alpha

• Entrez Gene IDs:

  6095 (Human)

• Alternate Names:

  DKFZp686M2414; MGC119326; NR1F1; NR1F1MGC119329; nuclear receptor ROR-alpha; Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; RAR-related orphan receptor A; RAR-related orphan receptor alpha; retinoic acid receptor-related orphan receptor alpha; retinoid-related orphan receptor alpha; Retinoid-related orphan receptor-alpha; ROR alpha; ROR1; ROR2; ROR3; RORA; RZRA; RZR-ALPHA; RZRAROR-alpha; transcription factor RZR-alpha