详细说明
- Species ReactivityHuman
- SpecificityDetects human VEGF 165b in direct ELISAs and Western blots. Recognizes an epitope within a 9 amino acid sequence at the C-terminus of human VEGF 165b. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) VEGF 206, rhVEGF-B 167, rhVEGF-B 186, rhVEGF-C, or rhVEGF-D is observed.
- SourceMonoclonal Mouse IgG 1 Clone # 56-1
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenKLH-conjugated human VEGF 165b synthetic peptide
TCRSLTRKD
Accession # AAL27435 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
Applications
- Recommended
ConcentrationSample
- Western Blot1 µg/mLRecombinant Human VEGF 165b (Catalog # )
- ImmunohistochemistryBates, D.O. et al. (2006) Clinical Sci. 110:575.
- ImmunofluorescenceWoolard, J. et al. (2004) Canc. Res. 64:7822.
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
| Immunohistochemistry | VEGF165b in Human Placenta. VEGF165b was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human VEGF165b Monoclonal Antibody (Catalog # MAB3045) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for . |
Preparation and Storage
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGF
VEGF 165b is the protein resulting from an mRNA splice variant of VEGF. Alternative exon usage results in a 165 amino acid product that is identical to VEGF 165 except for 6 unique amino acids at the C-terminus. VEGF 165b inhibits proliferation and migration of endothelial cells and is down-regulated in renal cell carcinomas.
- Long Name:Vascular Endothelial Growth Factor
- Entrez Gene IDs:7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
- Alternate Names:MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor
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