• Species Reactivity

  Human

• Specificity

  Detects human BMP-2 and human BMP-4 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows no cross‑reactivity with recombinant human (rh) BMP-5 or rhBMP-6.

• Source

  Monoclonal Mouse IgG    _2B Clone # 65529

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human BMP-2    
  Ala284-Arg396    
  Accession # P12643

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human BMP‑2 (Catalog # )    
  Recombinant Human BMP‑4 (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-2/BMP-4

BMP-2 and BMP-4 belong to a family of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF-beta ) superfamily, BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs.

• Long Name:

  Bone Morphogenetic Protein 2/4

• Entrez Gene IDs:

  650 (Human); 12156 (Mouse); 29373 (Rat)

• Alternate Names:

  BDA2; BMP-2; BMP-2/BMP-4; BMP2A; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2