• Species Reactivity

  Human

• Specificity

  Conjugated LAMP2/CD107b antibodies are ideal for immunocytochemistry colocalization studies in lysosomes. The unconjugated antibody detects human LAMP2/CD107b in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human LAMP1 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 743320

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human LAMP2/CD107b    
  Leu29-Phe375    
  Accession # P13473

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 488

Applications

• Recommended    
  Concentration

  Sample

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

• Intracellular Staining by Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LAMP-2/CD107b

Lysosomal associated membrane protein 2 (LAMP2), also known as CD107b and LGP110, is an approximately 110 kDa transmembrane glycoprotein that is a major component of lysosomal membranes (1). Mature human LAMP2 consists of a 347 amino acid (aa) intralumenal domain, a 24 aa transmembrane segment, and a 35 aa cytoplasmic tail (2). Its lumenal domain is organized into two heavily N-glycosylated regions separated by a Ser/Pro-rich linker that carries a minor amount of O‑linked glycosylation (2, 3). Alternate splicing generates a human LAMP2 isoform (LAMP2B) with a substituted juxtamembrane lumenal region, transmembrane segment, and cytoplasmic tail (4). Within the lumenal domain, human LAMP2 shares approximately 64% aa sequence identity with mouse and rat LAMP2. LAMP2 itself is subject to lysosomal degradation following cleavage of its lumenal domain (5). It mediates the lysosomal uptake of the chaperone HSC73 in complex with cargo proteins and is required for the lysosomal destruction of autophagic vacuoles (6, 7). In cytotoxic T cells and mast cells, LAMP2 is expressed in the membranes of intracellular granules that contain effector molecules such as perforin, granzymes, eicosanoids, and histamine (8-10). Up‑regulated LAMP2 at the plasma membrane serves as an indicator of cell activation of CD8  ⁺ T cells, mast cells, monocytes, and platelets (9-12). LAMP2 is a native ligand for lectins Galectin-1 and Galectin-3 (13‑15).

• References:

1. Eskelinen, E.-L. et al. (2003) Trends Cell Biol. 13:137.

2. Fukuda, M. et al. (1988) J. Biol. Chem. 263:18920.

3. Carlsson, S.R. et al. (1988) J. Biol. Chem. 263:18911.

4. Konecki, D.S. et al. (1995) Biochem. Biophys. Res. Commun. 215:757.

5. Cuervo, A.M. and J.F. Dice (2000) Traffic 1:570.

6. Cuervo, A.M. and J.F. Dice (1996) Science 273:501.

7. Tanaka, Y. et al. (1990) Nature 406:902.

8. Peters, P.J. et al. (1991) J. Exp. Med. 173:1099.

9. Betts, M.R. et al. (2003) J. Immunol. Meth. 281:65.

10. Grutzkau, A. et al. (2004) Cytometry 61:62.

11. Kannan, K. et al. (1996) Cell. Immunol. 171:10.

12. Silverstein, R.L. and M. Febbraio (1992) Blood 80:1470.

13. Skrincosky, D.M. et al. (1993) Cancer Res. 53:2667.

14. Inohara, H. and Raz, A. (1994) Biochem. Biophys. Res. Commun. 201:1366.

15. Ohannesian D.W. et al. (1994) Cancer Res. 54:5992.

• Long Name:

  Lysosome-associated Membrane Glycoprotein 2

• Entrez Gene IDs:

  3920 (Human)

• Alternate Names:

  CD107 antigen-like family member B; CD107b antigen; CD107b; LAMP2; LAMP-2; LAMPB; LGP110; lysosomal-associated membrane protein 2; lysosome-associated membrane glycoprotein 2; Lysosome-associated membrane protein 2