详细说明
- SpecificityDetects N-terminal and C-terminal V5 epitope-tagged proteins in Western blots.
- SourceRecombinant Monoclonal Rabbit IgG Clone # 1036H
- PurificationProtein A or G purified from cell culture supernatant
- ImmunogenV5 epitope tag peptide
Accession # Y138513 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
- LabelUnconjugated
Applications
- Recommended
ConcentrationSample
- Western Blot0.1 µg/mLSee below
- Simple Western1 µg/mLSee below
- Flow Cytometry0.25 µg/10 6 cellsSee below
- Immunoprecipitation1-4 µg/100 µg cell lysateHEK293 human embryonic kidney cell line transfected with N-terminal V5-tagged FDNC4 and C-terminal V5-tagged EAA1, .
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
| Western Blot | Detection of V5 Epitope-tagged proteins by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal V5-tagged CBL, N-terminal V5-tagged FSNC4, C-terminal V5-tagged LAMP2B, and C-terminal V5-tagged GALNT2. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-V5 Epitope Tag Monoclonal Antibody (Catalog # MAB8926) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # ). Specific bands were detected for V5 Epitope-tagged at approximately 110 kDa, 34 kDa, 75 kDa, and 65 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
| Flow Cytometry | Detection of V5 Epitope Tag in HEK293 Human Cell Line Transfected with V5-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with V5-tagged proteins was stained with Rabbit Anti-V5 Epitope Tag Monoclonal Antibody (Catalog # MAB8926, filled histogram) or isotype control antibody (Catalog # , open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # ). |
| Simple Western | Detection of V5 Epitope Tag by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal V5-tagged CBL and C-terminal V5-tagged GALNT2, loaded at 0.2 mg/mL. Specific bands were detected for V5 Epitope-tagged proteins at approximately 119 kDa and 65 kDa (as indicated) using 1 µg/mL of Rabbit Anti-V5 Epitope Tag Monoclonal Antibody (Catalog # MAB8926). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
Preparation and Storage
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: V5 Epitope Tag
- Entrez Gene IDs:3160800 (Viral)
- Alternate Names:GKPIPNPLLGLDST Epitope Tag; Parainfluenza virus 5 V/P; Paramyxovirus SV5; V5 Epitope Tag
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