Human EN-RAGE MAb (Clone 161212) 100 UG

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Human EN-RAGE MAb (Clone 161212) 100 UG信息二维码

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  • 更新2024-11-27 01:04
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产品介绍

    基本参数

    详细说明

    • Species Reactivity

      Human

    • Specificity

      Detects human EN-RAGE/S100A12 in direct ELISAs and Western blots.

    • Source

      Monoclonal Mouse IgG    2A Clone # 161212

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      E.coli-derived recombinant human EN-RAGE/S100A12    
      Met1-Glu92    
      Accession # NP_005612

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      2 µg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Western Blot      
         

    Detection of Human EN‑RAGE/S100A12 by Western Blot. Western blot shows lysates of human granulocytes. PVDF Membrane was probed with 2 µg/mL of Human EN‑RAGE/S100A12 Monoclonal Antibody (Catalog # MAB10522) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for EN‑RAGE/S100A12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using .

    Preparation and Storage

    • Reconstitution

      Sterile PBS to a final concentration of 0.5 mg/mL.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: EN-RAGE/S100A12

    EN-RAGE, also known as S100A12 and Calgranulin C, is a 10 kDa secreted molecule that belongs to the S100 family of calcium-binding proteins. EN-RAGE contains two EF-hand domains and forms dimers and hexamers in solution. EN-RAGE is overexpressed at sites of inflammation and interacts with RAGE on endothelial cells, lymphocytes, and monocytes to promote inflammatory responses. Human EN-RAGE shares 66% and 70% amino acid sequence identity with bovine and porcine EN-RAGE, respectively; mouse and rat orthologs have not been identified.

    • Long Name:

      Extracellular Newly Identified RAGE-binding Protein

    • Entrez Gene IDs:

      6283 (Human)

    • Alternate Names:

      CAAF1; CAAF1Neutrophil S100 protein; CAAFI; CAGC; CAGCS100 calcium binding protein A12 (calgranulin C); CAGCS100; Calcium-binding protein in amniotic fluid 1; Calgranulin C; calgranulin-C; CGRPEN-RAGE; ENRAGE; EN-RAGE; Extracellular newly identified RAGE-binding protein; MRP6; p6calgranulin C; protein S100-A12; S100 calcium binding protein A12; S100 calcium-binding protein A12 (calgranulin C); S100 calcium-binding protein A12; S100A12





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