详细说明
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Species Reactivity
Human
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Specificity
Detects human Mesothelin Propeptide/MPF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Mesothelin is observed.
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Source
Monoclonal Mouse IgG 1 Clone # 346633
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Purification
Protein A or G purified from hybridoma culture supernatant
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Immunogen
Mouse myeloma cell line NS0-derived recombinant human Mesothelin propeptide
Arg35-Leu289
Accession # AAH09272
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
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Label
Unconjugated
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
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Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
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Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mesothelin
Megakaryocyte Potentiating Factor (MPF) is a 33 kDa soluble protein that is cleaved as an N-terminal propeptide from the mouse mesothelin preproprotein by furin, while the C-terminal portion is processed further to form mesothelin. Mouse MPF potentiates megakaryocyte colony forming activity of IL-3 in mouse bone marrow culture. Human MPF shows 55% amino acid identity with mouse or rat MPF. The preproprotein is produced by mesothelial cells and is also highly expressed in pancreatic, gastric and ovarian carcinomas.
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Entrez Gene IDs:
10232 (Human); 56047 (Mouse); 60333 (Rat)
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Alternate Names:
CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein