详细说明
Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Glucagon.
Source
Monoclonal Mouse IgG 2A Clone # 181402
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Glucagon synthetic peptide
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488
Applications
Recommended
ConcentrationSample
Intracellular Staining by Flow Cytometry
0.25-1 µg/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry | Detection of Glucagon in beta TC-6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Alexa Fluor® 488-conjugated Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # IC1249G, filled histogram) or isotype control antibody (Catalog # , open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Glucagon
Glucagon is a 29 amino acid residue pancreatic peptide hormone. It stimulates gluconeogenesis and glycogenolysis to counter the glucose-lowering action of insulin.
Entrez Gene IDs:
2641 (Human); 14526 (Mouse); 24952 (Rat)
Alternate Names:
GCG; glicentin-related polypeptide; Glucagon; GRPP