• Species Reactivity

  Mouse

• Specificity

  Detects mouse CD36/SR-B3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD36 is observed.

• Source

  Monoclonal Rat IgG1 Clone # 324205

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant mouse CD36/SR-B3    
  Gly30-Lys439    
  Accession # Q08857

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 350

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  J774A.1 mouse reticulum cell sarcoma macrophage cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD36/SR-B3

CD36 (alternatively known as platelet membrane glycoprotein IV (GPIV), thrombospondin receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3)) is an 88 kDa, integral membrane glycoprotein that belongs to the class B scavenger receptor family (1, 2). The molecule is described as being ditopic, with two transmembrane segments connected by an extracellular loop (3). Mouse CD36 is synthesized as a 472 amino acid (aa) protein that contains a 6 aa N‑terminal cytoplasmic domain, a 22 aa N‑terminal transmembrane segment, a 420 aa extracellular “loop”, a 22 aa C‑terminal transmembrane segment, and a 9 aa C‑terminal cytoplasmic tail (4). Both cytoplasmic tails are palmitoylated, with the C‑terminal tail involved in oxidized LDL binding (5, 6). With respect to the extracellular loop, the N‑terminal region is believed to bind both thrombospondin-1 and Plasmodium-infected erythrocytes. Other ligands for CD36 include long-chain fatty acids, collagen, phospholipids and apoptotic cells (1). The extracellular loop of mouse CD36 shares 94%, 92%, 84% and 84% aa sequence identity with the extracellular loops of rat, hamster, human and bovine CD36, respectively. Cells known to express CD36 include capillary endothelium, adipocytes, skeletal muscle cells, intestinal epithelium, smooth muscle cells and hematopoietic cells such as RBC’s, platelets and monocytes (1). On the surface of cells, CD36 is suggested to exist as a dimer in response to ligation (7). CD36 is reported to regulate fatty uptake, act as an angiogenic with TSP-1, and participate in the clearance of apoptotic phagocytes (1, 8).

• References:

1. Febbraio, M. et al. (2001) J. Clin. Invest. 108:795. 

2. Silverstein, R.L. and M. Febbraio (2000) Curr. Opin. Lipid. 11:483. 

3. Gruarin, P. et al. (2000) Biochem. Biophys. Res. Commun. 275:446. 

4. Endemann, G. et al. (1993) J. Biol. Chem. 268:11811. 

5. Malaud, E. et al. (2002) Biochem. J. 364:507.

6. Tao, N. et al. (1996) J. Biol. Chem. 271:22315.

7. Daviet, L. et al. (1997) Thromb. Haemost. 78:897.

8. Simantov, R. and R.L. Silverstein (2003) Front. Biosci. 8:s874.

• Long Name:

  Scavenger Receptor Class B, Member 3

• Entrez Gene IDs:

  948 (Human); 12491 (Mouse)

• Alternate Names:

  CD36 antigen; CD36 molecule (thrombospondin receptor); CD36; Collagen R; FAT; FATCHDS7; Fatty acid translocase; Glycoprotein IIIb; GP3Bthrombospondin receptor); GPIIIb; GPIV; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; Platelet collagen receptor; platelet glycoprotein 4; Platelet glycoprotein IV; SCARB3; scavenger receptor class B, member 3; SRB3; SR-B3; Thrombospondin R; Thrombospondin receptor