详细说明
- Species ReactivityHuman
- SpecificityDetects human Tryptase epsilon /BSSP-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Tryptase epsilon /BSSP-4 is observed.
- SourceMonoclonal Mouse IgG 2A Clone # 725445
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenMouse myeloma cell line NS0-derived recombinant human Tryptase epsilon /BSSP-4
Ala33-Ser317
Accession # Q9GZN4 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
Applications
- Recommended
ConcentrationSample
- Western Blot2 µg/mLSee below
- Immunohistochemistry8-25 µg/mLSee below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
| Western Blot | Detection of Human Tryptase epsilon /BSSP‑4 by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Tryptase epsilon /BSSP‑4 Monoclonal Antibody (Catalog # MAB5047) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for Tryptase epsilon /BSSP‑4 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
| Immunohistochemistry | Tryptase epsilon /BSSP‑4 in Human Pancreas. Tryptase epsilon /BSSP‑4 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Tryptase epsilon /BSSP‑4 Monoclonal Antibody (Catalog # MAB5047) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # ). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in exocrine cells. View our protocol for . |
Preparation and Storage
- ReconstitutionSterile PBS to a final concentration of 0.5 mg/mL.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tryptase epsilon/BSSP-4
Tryptase epsilon, also known as brain-specific protease-4 (BSSP-4) and prosemin, is expressed in the brain and epithelium-rich tissues such as the lung and eye (1, 2). BSSP-4 is a serine protease related to trypsin, preferentially hydrolyzing substrates after arginine and lysine residues. However, Tryptase epsilon is less susceptible to inhibition by common trypsin inhibitors such as aprotinin, alpha 1-antitrypsin and secretory leukocyte protease inhibitor (3). Tryptase epsilon efficiently converts pro-urokinase-type plasminogen activator to its mature, active form (3).
- References:
- Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.
- Wong, G.W. et al. (2001) J. Biol. Chem. 276:49169.
- Yasuda, S. et al. (2005) Blood 105:3893.
- Entrez Gene IDs:64063 (Human); 70835 (Mouse)
- Alternate Names:BSP-2; BSSP4; BSSP-4; hBSSP-4; MGC9599; prosemin; protease, serine S1 family member 22; protease, serine, 22; PRSS22; serine protease 22; serine protease 26; SP001LA; Tryptase epsilon; UNQ302/PRO343
4006226992