• Species Reactivity

  Human

• Specificity

  Detects human CD1b in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD1a, rhCD1c, rhCD1d, rhCD1e, recombinant mouse (rm) CD1d1, or rmCD1d2 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 737249

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human CD1b    
  Ser18-Ser303    
  Accession # P29016

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD1b

CD1b is an approximately 40-50 kDa transmembrane glycoprotein in the CD1 family of glycolipid antigen-presenting MHC-like molecules. CD1b associates with beta 2-microglobulin in endosomes and the Golgi where it facilitates the processing of glycolipids for their presentation by other CD1 family proteins. CD1b is expressed by most nonhuman mammals but not by mice or rats. It contains one Ig-like domain in its extracellular region. Alternate splicing of human CD1b generates an additional isoform that lacks 55 amino acids of the Ig-like domain.

• Entrez Gene IDs:

  910 (Human)

• Alternate Names:

  CD1; CD1A; CD1b antigen; CD1B antigen, b polypeptide; CD1b molecule; CD1b; cortical thymocyte antigen CD1B; differentiation antigen CD1-alpha-3; MGC125990; MGC125991; R1; T-cell surface glycoprotein CD1b