• Species Reactivity

  Human

• Specificity

  Detects human TLE2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human TLE1, 3, or 4 is observed.

• Source

  Monoclonal Mouse IgG    _2A Clone # 737428

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human TLE2    
  Leu11-Ser193    
  Accession # Q04725

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLE2

TLE2 (Transducin-Like Enhancer of Split 2; also ESG2 and Grg-2) is an 83-84 kDa member of the WD repeat Groucho/TLE family of transcriptional repressors. It is ubiquitously expressed and is known to antagonize TCF (Wnt)-mediated signaling. TLE2 binds to other family members as a heterooligomer or to itself as a homooligomer. While it possesses no intrinsic DNA-binding activity, it does modulate the activity of multiple factors such as FoxG1, Arx and histone H3. Human TLE2 is 743 amino acids (aa) in length. It contains a Gln-rich region that mediates oligomerization (aa 1-133), a CCN domain that contains an NLS (aa 195-256), and six WD repeats that mediate protein-protein interaction (aa 455-742). There are at least five potential phosphorylation sites that, if used, may account for SDS-PAGE MWs exceeding 95 kDa. Potential isoform variants exist. One possesses a 21 aa substitution for aa 1-9, while a second shows the same substitution coupled to an additional 10 aa substitution for aa 683-743. A third isoform possesses an alternative start site at Met56 coupled to a deletion of aa 124-190. Over aa 11-193, human TLE2 shares 91% aa identity with mouse TLE2.

• Long Name:

  Transducin-like Enhancer of Split 2

• Entrez Gene IDs:

  7089 (Human); 21886 (Mouse); 299636 (Rat)

• Alternate Names:

  enhancer of split groucho 2; ESG; ESG2; ESG2Enhancer of split groucho-like protein 2; FLJ41188; GRG2; TLE2; transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 2, homolog of Drosophila E(sp1); transducin-like enhancer protein 2