详细说明
Species Reactivity
Mouse
Specificity
Stains mouse CXCR2-transfected cells but not CXCR1 transfectants.
Source
Monoclonal Rat IgG 2A Clone # 242216
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
C6 rat glioma cell line transfected with mouse CXCR2
Met1-Leu359
Accession # P35343Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of CXCR2/IL‑8 RB in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Gr‑1/Ly‑6G Fluorescein‑conjugated Monoclonal Antibody (Catalog # ) and either (A) Rat Anti-Mouse CXCR2/IL‑8 RB APC‑conjugated Monoclonal Antibody (Catalog # FAB2164A) or (B) Rat IgG1 Allophycocyanin Isotype Control (Catalog # ). View our protocol for . |
Flow Cytometry | Detection of CXCR2/IL‑8 RB in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouse CXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL‑8 RB APC‑conjugated Monoclonal Antibody (Catalog # FAB2164A). Quadrant markers were set based on control antibody staining (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCR2/IL-8 RB
CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.
Long Name:
Interleukin 8 Receptor B
Entrez Gene IDs:
3579 (Human); 12765 (Mouse); 29385 (Rat)
Alternate Names:
beta; CD182 antigen; CD182; CDw128b; chemokine (C-X-C motif) receptor 2; chemokine (CXC) receptor 2; C-X-C chemokine receptor type 2; CXCR2 gene for IL8 receptor type B; CXCR2; CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8 receptor type 2; IL-8R B; IL8R2; IL8RA; IL8RB; IL-8RB; interleukin 8 receptor B; interleukin 8 receptor type 2; interleukin-8 receptor type B