• Species Reactivity

  Human, Mouse

• Specificity

  Detects human and mouse SOX2 in Western blots.

• Source

  Monoclonal Mouse IgG    _2A Clone # 245610

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human SOX2    
  Gly135-Met317    
  Accession # P48431

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SOX2

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).

• References:

1. Graham, V. et al. (2003) Neuron 39:749.

2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.

3. Kishi, M. et al. (2000) Development 127:791.

4. Yuan, H. et al. (1995) Genes Dev. 9:2635.

5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.

6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.

• Long Name:

  Transcription Factor SOX2

• Entrez Gene IDs:

  6657 (Human)

• Alternate Names:

  ANOP3; MCOPS3; MGC2413; SOX2; SRY (sex determining region Y)-box 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2