详细说明
- Species ReactivityHuman
- SpecificityDetects human N-Cadherin propeptide in direct ELISAs. Shows no cross-reactivity with recombinant human (rh) Cadherin-4, -6, -8, -11, -12,
-13, -17, rhE-Cadherin, rhP-Cadherin and rhVE-Cadherin. - SourceMonoclonal Rat IgG 2A Clone # 401408
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenE. coli-derived recombinant human N-Cadherin propeptide
Pro26-Lys159
Accession # P10922 - FormulationSupplied in a saline solution containing BSA and Sodium Azide.
- LabelPhycoerythrin
- Recommended
ConcentrationSample
- Intracellular Staining by Flow Cytometry10 µL/10 6 cellsSee below
Intracellular Staining by Flow Cytometry | Detection of N‑Cadherin Propeptide in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human N‑Cadherin Propeptide PE‑conjugated Monoclonal Antibody (Catalog # IC1388P, filled histogram) or isotype control antibody (Catalog # , open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for . |
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageProtect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
N‑Cadherin (Neural Cadherin; also CD325 and Cadherin-2) is a 130‑135 kDa member of the "classical" (or type I) cadherin subfamily, cadherin superfamily of proteins. It is expressed on multiple cell types, including neurons, fibroblasts, Schwann cells, endothelial cells and hepatic stellate cells. N‑Cadherin mediates homotypic binding, either in cis (same cell) or trans (adjacent cell). proN‑Cadherin is expressed as an 881 amino acid (aa) type I transmembrane glycoprotein. It may be initially inserted into the ER, where the 15‑20 kDa prodomain (aa 26‑159) is cleaved by proprotein convertase, and the mature molecule is transported to the surface. Alternatively, on neurons, proN‑Cadherin may first appear on the surface, with cleavage occurring at the time of synaptogenesis. Cleavage appears necessary for homophilic interaction as presence of the prodomain is suggested to negatively regulate oligomer formation. Over the entire prodomain, the human N‑Cadherin proregion shares 87% aa identity with the mouse N‑Cadherin proregion.
- Long Name:Neural Cadherin
- Entrez Gene IDs:1000 (Human); 12558 (Mouse); 83501 (Rat)
- Alternate Names:cadherin 2, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; CD325 antigen; CD325; CDH2; CDHNcalcium-dependent adhesion protein, neuronal; CDw325; NCadherin; N-Cadherin; NCADN-cadherin 1; Neural cadherin; neural-cadherin