• Species Reactivity

  Human

• Specificity

  Detects human CD53. Stains human CD53 transfectants but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG    _2A Clone # 425514

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human CD53    
  Met1-Leu219    
  Accession # P19397

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Allophycocyanin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD53

CD53, also known as TSPAN25, is a 35 kDa‑40 kDa cell surface and endosomal membrane glycoprotein that belongs to the 4 TM tetraspanin superfamily of molecules. CD53 is widely expressed on hematopoietic cells, including eosinophils, neutrophils, mast cells, CD16⁺ proinflammatory monocytes, NK cells T cells, macrophages, splenic B cells and immature dendritic cells. Ligation of CD53 promotes cell proliferation and survival, as well as homotypic cell‑cell adhesion. On the cell membrane, CD53 associates with other tetraspanins, MHC class II molecules, and alpha 3 and alpha 4 integrins. Human CD53 shares 71% aa sequence identity with mouse and CD53 in the extracellular domains.

• Entrez Gene IDs:

  963 (Human); 12508 (Mouse); 24251 (Rat)

• Alternate Names:

  antigen MOX44 identified by monoclonal MRC-OX44; CD53 antigentetraspanin-25; CD53 glycoprotein; CD53 molecule; CD53 tetraspan antigen; CD53; cell surface antigen; Cell surface glycoprotein CD53; MOX44; MOX44transmembrane glycoprotein; Tetraspanin-25; TSPAN25; tspan-25; TSPAN25leukocyte surface antigen CD53