详细说明
- Species ReactivityHuman
- SpecificityDetects human Peptide YY.
- SourceRecombinant Monoclonal Rabbit IgG Clone # 2163A
- PurificationProtein A or G purified from cell culture supernatant
- ImmunogenHuman Peptide YY synthetic peptide
Accession # P10082 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
Applications
- Recommended
ConcentrationSample
- Immunohistochemistry3-10 µg/mLSee below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
| Immunohistochemistry | Peptide YY in Human Pancreas. Peptide YY was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human Peptide YY Monoclonal Antibody (Catalog # MAB9456) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # ). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in islet cells. View our protocol for . |
Preparation and Storage
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Peptide YY
Peptide YY (PYY), a secretory peptide, is expressed by L cells (enteroendocrine cells) in the small and large intestine, Islet of Langerhans cells, testis, and in some central and peripheral neurons. Because PYY is released from L cells after feeding, it is thought to produce a satiety signal. While the regulation of appetite by PYY and GLP-1 have been studied extensively, recent evidence supports a role for PYY in Islet of Langerhans beta cell survival. PYY secretion is also modulated by gut microbiota, and levels of colonic PYY have reduced in rats with DSS-induced colitis, and in humans with inflammatory bowel disease (IBD), while circulating PYY increased in IBD.
- Entrez Gene IDs:5697 (Human); 217212 (Mouse); 287730 (E. faecalis)
- Alternate Names:Peptide tyrosine tyrosine; Peptide YY; Peptide-YY; PYY; PYY1; PYY-I
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