• Species Reactivity

  Human

• Specificity

  Detects human MRP1 in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # QCRL

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  H69AR human small cell lung cancer cell line membrane extracts

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  Hipfner, D.R.     et al. (1994) Canc. Res.     54:5788; Hipfner, D.R.     et al. (1996) Canc. Res.     56:3307. This application was not tested by R&D Systems.

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• Immunoprecipitation

  Hipfner, D.R.,     et al. (1994) Canc. Res.     54:5788.

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• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Immunocytochemistry

  8-25 µg/mL

  Immersion fixed H69AR human small cell lung cancer cell line

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• Intracellular Staining by Flow Cytometry

  2.5 µg/10    ⁶ cells

  H69AR human small cell lung cancer cell line fixed with paraformaldehyde and permeabilized with saponin

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MRP1

MRP1 (Multidrug Resistance associated Protein 1), also known as ABCC1, belongs to the family of ATP-Binding Cassette (ABC) transporters. It confers multidrug resistance on tumor cells and is overexpressed in various human multidrug resistant tumor cell lines.

• Long Name:

  Multi-drug Resistance Protein 1

• Entrez Gene IDs:

  4363 (Human); 17250 (Mouse); 24565 (Rat)

• Alternate Names:

  ABC29; ABCC; ABCC1; ATP-binding cassette sub-family C member 1; ATP-binding cassette, sub-family C (CFTR/MRP), member 1; EC 3.6.3; EC 3.6.3.44; GS-X; Leukotriene C(4) transporter; LTC4 transporter; MRP1; MRP1DKFZp781G125; MRPDKFZp686N04233; multidrug resistance associated protein 1; multidrug resistance protein; multidrug resistance-associated protein 1; multiple drug resistance protein 1; multiple drug resistance-associated protein