详细说明
Species Reactivity
Human
Specificity
Detects human TRA‑1‑85 antigen in Western blots.
Source
Monoclonal Mouse IgG 1 Clone # TRA-1-85
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
2120Ep human embryonal carcinoma cell line
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/10 6 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
8-25 µg/mL
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Western Blot | Detection of Human TRA‑1‑85/CD147 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human heart tissue, and human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TRA‑1‑85/CD147 Monoclonal Antibody (Catalog # MAB3195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for TRA‑1‑85/CD147 at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Flow Cytometry | Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA‑1‑85/CD147 Monoclonal Antibody (Catalog # MAB3195) or (B) Mouse IgG1 Isotype Control (Catalog # ) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). |
Immunocytochemistry | TRA‑1‑85/CD147 in BG01V Human Embryonic Stem Cells. TRA‑1‑85/CD147 was detected in immersion fixed BG01V human embryonic stem cells on irradiated mouse embryonic fibroblasts using Mouse Anti-Human TRA‑1‑85/CD147 Monoclonal Antibody (Catalog # MAB3195) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for . |
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRA-1-85/CD147
The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).
References:
Williams, B.P. et al. (1988) Immunogenetics. 27:322.
Spring, F.A. et al. (1997) Eur. J. Immunol. 27:891.
Entrez Gene IDs:
682 (Human)
Alternate Names:
OKa Blood Group Antigen; TRA185; TRA-1-85