Human TRA-1-85/CD147 MAb (Clone TRA-1-85) 100 UG

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Human TRA-1-85/CD147 MAb (Clone TRA-1-85) 100 UG信息二维码

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产品介绍

    基本参数

    详细说明

    • Species Reactivity

      Human

    • Specificity

      Detects human TRA‑1‑85 antigen in Western blots.

    • Source

      Monoclonal Mouse IgG    1 Clone # TRA-1-85

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      2120Ep human embryonal carcinoma cell line

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      1 µg/mL

      See below


    • Flow Cytometry

      0.25 µg/10    6 cells

      See below


    • CyTOF-ready

      Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.


    • Immunocytochemistry

      8-25 µg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Western Blot      
         

    Detection of Human TRA‑1‑85/CD147 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human heart tissue, and human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TRA‑1‑85/CD147 Monoclonal Antibody (Catalog # MAB3195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for
    TRA‑1‑85/CD147 at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using .

    Flow Cytometry      
         

    Detection of TRA‑1‑85/CD147 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA‑1‑85/CD147 Monoclonal Antibody (Catalog # MAB3195) or (B) Mouse IgG1 Isotype Control (Catalog # ) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ).

    Immunocytochemistry      
         

    TRA‑1‑85/CD147 in BG01V Human Embryonic Stem Cells. TRA‑1‑85/CD147 was detected in immersion fixed BG01V human embryonic stem cells on irradiated mouse embryonic fibroblasts using Mouse Anti-Human TRA‑1‑85/CD147 Monoclonal Antibody (Catalog # MAB3195) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for .

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.5 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: TRA-1-85/CD147

    The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).

    • References:

      1. Williams, B.P. et al. (1988) Immunogenetics. 27:322.

      2. Spring, F.A. et al. (1997) Eur. J. Immunol. 27:891.

    • Entrez Gene IDs:

      682 (Human)

    • Alternate Names:

      OKa Blood Group Antigen; TRA185; TRA-1-85







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