详细说明
- Species ReactivityHuman, Mouse
- SpecificityDetects human RND3 in direct ELISAs and Western blots.
- SourceMonoclonal Mouse IgG 1 Clone # 687202
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenRND3 (RD1 peptide)
Cys197-Arg207
Accession # P61587 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
Applications
- Recommended
ConcentrationSample
- Western Blot1 µg/mLSee below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
| Western Blot | Detection of Human RND3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, SH‑SY5Y human neuroblastoma cell line, MCF‑7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human RND3 (RD1 Peptide) Monoclonal Antibody (Catalog # MAB6618) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for RND3 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Preparation and Storage
- ReconstitutionSterile PBS to a final concentration of 0.5 mg/mL.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RND3
RND3, also known as RhoE, is an approximately 27 kDa member of the Rho family, small GTPase superfamily of molecules. It is 244 amino acids (aa) in length, and the mature chain extends from aa 1-241. RND3 is ubiquitously expressed and is involved in keratinocyte differentiation and stratification. RND3 overexpression results in cell size enlargement and an increase in the number of stratified cells. RND3 depletion results in hyperproliferation and delays initiation of keratinocyte differentiation. Human RND3 shares 100% aa sequence identity with mouse RND3.
- Long Name:Rho family GTPase 3
- Entrez Gene IDs:390 (Human)
- Alternate Names:ARHE; MemB; Protein MemB; Rho family GTPase 3member E; Rho8; RHOE; Rho-related GTP-binding protein Rho8; rho-related GTP-binding protein RhoE; RND3; small GTP binding protein Rho8
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