• Species Reactivity

  Human

• Specificity

  Detects human and mouse PRAS40 when phosphorylated at T246.

• Source

  Recombinant Monoclonal Rabbit IgG Clone # 1211A

• Purification

  Protein A or G purified from cell culture supernatant

• Immunogen

  Phosphopeptide containing the human PRAS40 T246 site    
  Accession # Q96B36

• Formulation

  Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1:1000 dilution

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 °C, as supplied.

• 1 month, 2 to 8 °C under sterile conditions after opening.

• 6 months, -20 to -70 °C under sterile conditions after opening.

Background: PRAS40

PRAS40 (Proline-rich AKT1 substrate 1), also known as Akt1S1 and p39, is a 40-42 kDa cytoplasmic phosphoprotein that lacks generally recognized structural motifs. It is widely expressed and is considered to be key regulator of mTORC1 (mTOR plus Raptor and G beta L), a complex through which Akt signals into the cell. Through phosphorylation, mTORC1 activity is upregulated by PRAS40. In particular, nonphosphorylated PRAS40 binds to and serves as a negative regulator of mTORC1 activity. Upon Insulin signaling, PRAS40 is phosphorylated on Thr246, Ser221 and Ser183. This causes it to bind 14-3-3 and results in its dissociation from mTORC1, freeing up mTOR to regulate (positively or negatively) protein synthesis. Human PRAS40 is 256 amino acids (aa) in length. It contains one extended Pro-rich region (aa 35-96) plus at least nine utilized Ser/Thr phosphorylation sites. There is one alternative start site at Met131. Over aa 119-256, human PRAS40 shares 93% aa sequence identity with mouse PRAS40.

• Long Name:

  40 kDa Proline-rich Akt1 Substrate

• Entrez Gene IDs:

  84335 (Human); 67605 (Mouse); 292887 (Rat)

• Alternate Names:

  40 kDa; AKT1 substrate 1 (proline-rich); AKT1S1; Lobe; MGC2865; PRAS40; proline-rich AKT1 substrate 1,40 kDa proline-rich AKT substrate