• Species Reactivity

  Human

• Specificity

  Detects human p70 S6 Kinase/p85 S6 Kinase when phosphorylated at T389/T412, respectively.

• Source

  Recombinant Monoclonal Rabbit IgG Clone # 1045C

• Purification

  Protein A or G purified from cell culture supernatant

• Immunogen

  Phosphopeptide containing the human p70 S6 Kinase T389 site

• Formulation

  Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1:1000 dilution

  See below

• 
  

• Immunocytochemistry

  1:50 dilution

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

• 12 months from date of receipt, -20 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after opening.

• 6 months, -20 °C under sterile conditions after opening.

Background: p70 S6 Kinase

p70 S6 Kinase (p70S6K) is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues (1). p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K activity undergoes changes in the cell cycle and increases  
20-fold in G1 cells released from G0 (2). p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as T389, a site phosphorylated by Phosphoinositide-Dependent Kinase 1 (PDK1).

• References:

1. Ferrari, S. et al. (1994) Crit. Rev. Biochem. Mol. Biol. 29:385.

2. Edelmann, H.M. et al. (1996) J. Biol. Chem. 271:963.

• Entrez Gene IDs:

  6198 (Human); 72508 (Mouse); 83840 (Rat)

• Alternate Names:

  EC 2.7.11; EC 2.7.11.1; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 Kinase; p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase, alpha 2; p70 S6KA; p70 S6K-alpha; p70(S6K)-alpha; p70-alpha; p70-S6K; P70S6K1; PS6K; ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kD, polypeptide 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; RPS6KB1; S6K; S6K1; S6K1p70-S6K 1; S6K-beta-1; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A; STK14A