Recombinant Human Sonic Hedgehog/Shh, N-Terminus GMP, CF 25 UG

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Recombinant Human Sonic Hedgehog/Shh, N-Terminus GMP, CF 25 UG信息二维码

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3-Amino-5-methoxycarbonylphenylboronic acid, pinacol ester  1 g 2,3-Dichloro-6-(trifluoromethyl)benzyl bromide  1 g 1-(4-Fluorophenyl)-5-methoxycarbonyl-2(1H)-pyridinone  1 g N,N-Diethyl-cyclohexane-1,4-diamine  1 g N-Methyl-DL-leucine hydrochloride  5 g N-(2-Chloroethyl) 3-boronobenzamide  5 g

产品介绍

    基本参数

    详细说明

    • Purity
      >97%, by SDS-PAGE with silver staining, under reducing conditions. Intact mass analysis of recombinant human sonic hedgehog confirms the predicted molecular mass of 20383 Da
    • Endotoxin Level
      <0.10 EU per 1 μg of the protein by the LAL method.
    • Activity
      Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED 50 for this effect is <5 μg/mL.
    • Source
      E. coli-derived Cys24-Gly197, with a C-terminal 6-His tag Produced using non-animal reagents in an animal-free laboratory.
      Manufactured and tested under cGMP guidelines.
    • Accession #
    • N-terminal Sequence
      Analysis
      (Cys 24)-Gly-Pro-Gly-Arg-Gly-Phe-Gly-Lys-Arg
    • Predicted Molecular Mass
      20 kDa
    • SDS-PAGE
      22 kDa, reducing conditions
    1314-GMP
     
    Formulation Lyophilized from a 0.2 μm filtered solution in NaH 2PO 4, NaCl and DTT.
    Reconstitution Reconstitute at 250 μg/mL in sterile deionized water.
    Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • A minimum of 6 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
    Data Images
    Bioactivity

    GMP-grade Recombinant Human Sonic Hedgehog/Shh, N-Terminus (Catalog # 1314-GMP) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 5 ug/mL.
    SDS-PAGE

    1 μg/lane of GMP-grade Recombinant Human Sonic Hedgehog/Shh, N-Terminus (Catalog # 1314-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.
    Background: Sonic Hedgehog/Shh
    Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Human Shh encodes a 462 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
    N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).
    • References:
      1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
      2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
      3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
      4. Marigo, V. et al. (1995) Genomics 28:44.
      5. Zeng, X. et al. (2001) Nature 411:716.
      6. Feng, J. et al. (2004) Development 131:4357.
      7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
      8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
      9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
      10. Etheridge, L.A. et al. (2010) Development 137:133.
      11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
      12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
      13. Lewis, P.M. et al. (2001) Cell 105:599.
      14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
      15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
      16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.
    • Entrez Gene IDs:
      6469 (Human); 20423 (Mouse)
    • Alternate Names:
      HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS

    END USER TERMS OF USE OF PRODUCT
    The following terms are offered to you upon your acceptance of these End User Terms of Use of Product. By using this product, you indicate your acknowledgment and agreement to these End User Terms of Use of Product. If you do not agree to be bound by and comply with all of the provisions of these End User Terms of Use of Product, you should contact your supplier of the product and make arrangements to return the product.

    We suggest you print and retain a copy of these End User Terms of Use of Product for your records.

    The End User is aware that R&D Systems, Inc. sells its GMP products for research use only or further manufacturing and not for in vivo use, the production of therapeutics or other drugs or for biologic products or devices. The End User further agrees, as a condition of the sale of R&D Systems'' GMP products that: a) the End User will not use this GMP Product in any procedure wherein the product may be directly or indirectly administered to humans, unless the End User has obtained, or prior to their use will have obtained, an Investigational New Drug (IND) exemption from the FDA and will use the product only in accordance with the protocols of such IND and of the Institutional Review Board overseeing the proposed research, or b) the End User will use the products outside of the United States in accordance with the protocols of research approved by the Institutional Review Board or authorized ethics committee and regulatory agencies to which the End User is subject to in their territory.

    R&D Systems, Inc. has the right, at its sole discretion, to modify, add or remove any terms or conditions of these End User Terms of Use without notice or liability to you. Any changes to these End User Terms of Use are effective immediately following the printing of such changes on this product insert. The most recent version of these End User Terms of Use of Product may be found at:

    You agree to review these End User Terms of Use of Product to ensure any subsequent use by you of R&D Systems'' GMP Products following changes to these End User Terms of Use of Product constitutes your acceptance of all such changes.

    TERMS AND CONDITIONS
    The following limitation applies to R&D Systems'' warranty and liability for damages: All products are warranted to meet R&D Systems'' published specifications when used under normal laboratory conditions.

    R&D SYSTEMS DOES NOT MAKE ANY OTHER WARRANTY OR REPRESENTATION WHATSOEVER, WHETHER EXPRESS OR IMPLIED, WITH RESPECT TO ITS PRODUCTS. IN PARTICULAR, R&D SYSTEMS DOES NOT MAKE ANY WARRANTY OF SUITABILITY, NONINFRINGEMENT, MERCHANTABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE.

    NOTWITHSTANDING ANY OTHER PROVISIONS OF THESE TERMS AND/OR ANY OTHER AGREEMENT BETWEEN R&D SYSTEMS AND PURCHASER FOR THE PURCHASE OF THE PRODUCTS, R&D SYSTEMS'' TOTAL LIABILITY TO PURCHASER ARISING FROM OR IN RELATION TO THESE TERMS, AN AGREEMENT BETWEEN THE PARTIES OR THE PRODUCTS, WHETHER ARISING IN CONTRACT, TORT OR OTHERWISE SHALL BE LIMITED TO THE TOTAL AMOUNT PAID BY PURCHASER TO R&D SYSTEMS FOR THE APPLICABLE PRODUCTS. IN NO EVENT WILL R&D SYSTEMS BE LIABLE FOR THE COST OF PROCUREMENT OF SUBSTITUTE GOODS.

    Full details of R&D Systems'' Terms and Conditions of Sale can be found online at:

     
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