• Species Reactivity

  Human

• Specificity

  Detects human CCL8/MCP-2 in ELISAs and Western blots. In ELISAs, this antibody shows less than 0.1% cross-reactivity with recombinant human (rh) CCL15 and rmCCL3.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 35509

• Purification

  Protein A or G purified from ascites

• Immunogen

  E. coli-derived recombinant human CCL8/MCP-2

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human CCL8/MCP‑2 (Catalog # )

• 
  

• Human CCL8/MCP-2 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  Human CCL8/MCP‑2 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Human CCL8/MCP‑2 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human CCL8/MCP-2 Protein (Catalog # )

• 
  

• Neutralization

  Measured by its ability to neutralize CCL8/MCP‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND     ₅₀) is typically 1-4 µg/mL in the presence of 0.25 µg/mL Recombinant Human CCL8/MCP‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL8/MCP-2

CCL8, also known as Monocyte Chemoattractant Protein 2 (MCP-2), is an inflammatory CC chemokine that attracts monocytes, eosinophils and basophils. It is produced by many cell types and signals through interactions with CCR1, CCR2, CCR3 and CCR5.

• Entrez Gene IDs:

  6355 (Human); 20307 (Mouse)

• Alternate Names:

  C-C motif chemokine 8; CCL8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; member 8 (monocyte chemotactic protein 2); monocyte chemoattractant protein 2; SCYA10; small-inducible cytokine A8