• Species Reactivity

  Human

• Specificity

  Detects human Granzyme A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Granzyme B, rhGranzyme H, recombinant mouse (rm) Granzyme D, or rmGranzyme G is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 356422

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Granzyme A    
  Cys26-Val262    
  Accession # P12544

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Granzyme A (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Granzyme A

Granzyme A is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is the most abundant protease in CTL and NK cells. It induces caspase‑independent cell death when introduced into target cells by perforin (1). Human granzyme A is synthesized as a 262 amino acid (aa) precursor with a signal peptide (aa 1‑26), a propeptide (aa 27‑28) and a mature chain
(aa 29‑262) (2). After being activated by lysyl endopeptidase removal of the propeptide, Granzyme A cleaves thioester substrates. Mature human granzyme A shares 71% amino acid sequence identity with mouse granzyme A.

• References:

1. Lieberman, J. and Z. Fan (2003) Curr. Opin. Immunol. 15:553.

2. Gershenfeld, H.K. et al. (1988) Proc. Natl. Acad. Sci. USA 85:1184.

• Entrez Gene IDs:

  3001 (Human); 14938 (Mouse); 266708 (Rat)

• Alternate Names:

  CTL Tryptase; CTLA3; CTLA3Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factorserine protease); Cytotoxic T-lymphocyte proteinase 1; EC 3.4.21; Fragmentin-1; granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3); Granzyme A; Granzyme-1; GZMA; H factor; Hanukkah factor; HF; HFSP; HFSPEC 3.4.21.78