• Species Reactivity

  Human

• Specificity

  Detects human PlGF in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human (rh) VEGF, rhPDGF‑AA, rhPDGF‑AB, or rhPDGF‑BB.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 37203

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human PlGF    
  Ala21-Arg149    
  Accession # P49763

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Human PlGF Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  Human PlGF Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Human PlGF Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human PlGF Protein (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PlGF

Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. As a result of alternative splicing, at least two PlGF mRNAs encoding monomeric PlGF precursors containing 149 and 170 amino acid residues have been described. The expression of PlGF is not widespread but has been detected in human umbilical vein endothelial cells, placenta, choriocarcinoma cell lines, and in renal cell carcinoma associated with angiogenesis. The PlGF proteins bind with high-affinity to VEGF R1/Flt-1 but not to VEGF R2/Flk-1.

• Long Name:

  Placenta Growth Factor

• Entrez Gene IDs:

  5228 (Human); 18654 (Mouse)

• Alternate Names:

  D12S1900; PGF; PGFL; placenta growth factor; placental growth factor; placental growth factor, vascular endothelial growth factor-related protein; PlGF; PlGF-2; PLGFplacental growth factor-like; SHGC-10760