详细说明
- Species ReactivityHuman, Mouse
- SpecificityDetects human and mouse Cathepsin L in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cathepsin B, C, L2, O, S, or X/Z/P is observed. In Western blots, both the pro and active forms of recombinant human and mouse Cathspsin L are recognized.
- SourceMonoclonal Rat IgG 2A Clone # 204101
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenMouse myeloma cell line NS0-derived recombinant human Cathepsin L
Glu113-Val333
Accession # P07711 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Recommended
ConcentrationSample
- Western Blot1 µg/mLSee below
- Immunohistochemistry5-25 µg/mLSee below
- Immunoprecipitation25 µg/mLConditioned cell culture medium spiked with Recombinant Human Cathepsin L (Catalog # ) or Recombinant Mouse Cathepsin L (Catalog # ), . This antibody did not immunoprecipitate the pro form of recombinant mouse Cathepsin L.
Western Blot | Detection of Recombinant Human and Mouse Cathepsin L by Western Blot. Western blot shows 100 ng of Recombinant Human Cathepsin L (Catalog # ), Recombinant Mouse Cathepsin L (Catalog # ), Recombinant Human Cathepsin V (Catalog # ), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (Catalog # ), Recombinant Human Cathepsin H (Catalog # ), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # ). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using .For natural samples, we recommend the use of Catalog # . |
Immunohistochemistry | Cathepsin L in Human Kidney. Cathepsin L was detected in immersion fixed paraffin-embedded sections of human kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for . |
Immunohistochemistry | Cathepsin L in Mouse Kidney. Cathepsin L was detected in immersion fixed frozen sections of mouse kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for . |
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- References:
- Goretzki, L. et al. (1992) FEBS Lett. 297:112.
- Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
- Gottesman, M.M. and F. Cabral (1981) Biochemistry 20:1659.
- Entrez Gene IDs:1514 (Human); 1514 (Human); 13039 (Mouse)
- Alternate Names:Cathepsin L; cathepsin L1; CATL; CTSL; CTSL1; CTSLEC 3.4.22.15; EC 3.4.22; FLJ31037; Major excreted protein; MEP