详细说明
- Species ReactivityMouse
- SpecificityDetects mouse Rae‑1 epsilon in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Rae-1 alpha, beta, gamma, or δ is observed. By flow cytometry, no cross-reactivity with mouse Rae-1 alpha or mouse Rae-1 gamma.
- SourceMonoclonal Rat IgG 2A Clone # 205001
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenBaF3 mouse pro-B cell line transfected with mouse Rae-1 epsilon
- FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- Endotoxin Level<0.10 EU per 1 μg of the antibody by the LAL method.
- LabelUnconjugated
- Recommended
ConcentrationSample
- Flow Cytometry2.5 µg/10 6 cellsSee below
- Blockade of Receptor-ligand InteractionIn a functional ELISA, 0.3-0.9 µg/mL of this antibody will block 50% of the binding of 125 ng/mL of Recombinant Mouse NKG2D Fc Chimera (Catalog # ) to immobilized Recombinant Mouse Rae-1 epsilon Fc Chimera (Catalog # ) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
- CyTOF-readyReady to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry | Detection of Rae‑1 epsilon in BaF3 Mouse Cell Line Transfected with Mouse Rae-1 epsilon and eGFP by Flow Cytometry. BaF3 mouse pro-B cell line transfected with (A) mouse Rae-1 epsilon or (B) mouse Rae-1 alpha and eGFP was stained with Rat Anti-Mouse Rae‑1 epsilon Monoclonal Antibody (Catalog # MAB1135) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # ). Quadrant markers were set based on control antibody staining (Catalog # ). |
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Rae-1 epsilon is a member of a family of cell-surface proteins that function as ligands for mouse NKG2D. Other family members are designated Rae-1 alpha, beta, gamma, and δ. Amino acid sequence identity within this family ranges from 88‑95%. The Rae-1 proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig‑like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. The Rae-1 proteins are anchored to the membrane via a GPI‑linkage. The name of this family derives from the original identification of these proteins as the product of retinoic acid early inducible transcripts. Rae-1 expression is developmentally controlled. Transcripts were observed in the brain/head region of day 10‑14 embryos but disappeared by day 18. Rae-1 transcripts were detected in several transformed cell lines but are absent from most normal adult tissues. All Rae-1 family members bind to mouse NKG2D, an activating receptor expressed on NK cells and some T cell subsets, resulting in the activation of cytolytic activity and/or cytokine production by these effector cells. Ectopic expression of Rae-1 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1‑7).
- References:
- Zou, Z. et al. (1996) J. Biochem (Tokyo) 119:319.
- Diefenbach, A. et al. (2000) Nature Immunol. 1:119.
- Cerwenka, A. et al. (2000) Immunity 12:721.
- Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
- Diefenbach, A. et al. (2001) Nature 413:165.
- Champsaur, M. et al. (2010) J. Immunol. 185:157.
- Markiewicz M. et al. (2012) Immunity 36:132.
- Long Name:Retinoic Acid Early Transcript 1 epsilon
- Entrez Gene IDs:8480 (Human); 379043 (Mouse)
- Alternate Names:Rae1 epsilon; Rae-1 epsilon