• Species Reactivity

  Human

• Specificity

  Detects human CD9. Stains human CD9 transfectants, but not irrelevant transfectants in flow cytometry.

• Source

  Monoclonal Mouse IgG    _2B Clone # 209306

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human CD9    
  Met1-Val228    
  Accession # P21926

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  2.5 µg/10    ⁶ cells

  Human platelets

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• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD9

CD9, also known as motility-related protein-1 (MRP-1), is a tetraspan family glycoprotein expressed by a variety of hematopoietic and epithelial cells. It forms homotypic associations as well as heterotypic associations with other tetraspan proteins, some integrins and MHC class II proteins. CD9 has been shown to modulate cellular adhesion, migration and proliferation.

• Entrez Gene IDs:

  928 (Human); 12527 (Mouse)

• Alternate Names:

  BA2; BA-2/p24 antigen; BTCC-1; CD9 antigen; CD9 molecule; CD9; Cell growth-inhibiting gene 2 protein; DRAP-27; Leukocyte antigen MIC3; MIC3; motility related protein-1,5H9 antigen; Motility-related protein; MRP-1FLJ99568; p24; tetraspanin-29; TSPAN29; TSPAN-29; TSPAN29MIC3CD9 antigen (p24)