• Species Reactivity

  Human

• Specificity

  Detects human NOV/CCN3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse NOV, recombinant human (rh) CTGF, or rhWISP-1.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 209808

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human NOV/CCN3    
  Thr32-Met357    
  Accession # P48745

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human NOV/CCN3 (Catalog # )    
  under non-reducing conditions only

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NOV/CCN3

NOV, also called CCN3, is one of six CCN (CYR61/CTGF/NOV) secreted proteins which share a common multimodular organization (1 - 4). NOV/CCN3 contains an N-terminal IGFBP domain that appears to be non-functional and a vWF type C and thrombospondin type I domain which mediate oligomerization and matrix interactions, respectively (1, 2). The C-terminal cysteine knot domain interacts with several partners, including the matrix protein fibulin 1C (5), Notch-1 (6), and CCN2, with which it may heterodimerize (2). NOV/CCN3 also interacts with the gap junction protein Connexin43 and mediates suppression of proliferation (7). It also binds the calcium binding protein S100A4 and promotes calcium channel activation (8). The 357 amino acid (aa), 44 kDa human NOV/CCN3 shares 80% aa identity with mouse, rat and dog NOV/CCN3, and 78% aa identity with cow NOV/CCN3. NOV/CCN3 also shows 38 - 50% aa identity with other family members including WISP proteins, except for WISP-2/CCN5 which lacks the cysteine knot (1). NOV/CCN3 is widely expressed developmentally, especially in muscle, endothelium, nervous system, adrenal cortex and chondrocytes (1 - 4). In transformed cells, a 32 kDa N-terminally truncated form lacks the signal sequence is localized to the nucleus. Truncation allows a C-terminal nuclear localization sequence to be active (9). Nuclear NOV/CCN3 acts as a transcriptional repressor but promotes proliferation, presumably by interfering with growth control (9). Full length NOV/CCN3 is a secreted matricellular protein which inhibits cell growth. Interaction of NOV/CCN3 with integrins alpha _v beta ₃ and  alpha ₅ beta ₁ mediates endothelial cell adhesion, chemotaxis, and promotes angiogenesis (10, 11). Over-expression of NOV/CCN3 downregulates myogenic genes such as MyoD (12).

• References:

1. Perbal, B. (2004) Lancet 363:62.

2. Perbal, B. (2006) Cell Commun. Signal. 4:3.

3. Martinerie, C. et al. (1994) Oncogene 9:2729.

4. Snaith, M.R. et al. (1996) Genomics 38:425.

5. Perbal, B. et al. (1999) Proc. Natl. Acad. Sci. USA 96:869.

6. Sakamoto, K. et al. (2002) J. Biol. Chem. 277:29399.

7. Fu, C.T. et al. (2004) J. Biol. Chem. 279:36943.

8. Li, C.L. et al. (2002) Mol. Pathol. 55:250.

9. Planque, N. et al. (2006) J. Cell. Biochem. 99:105.

10. Lin, C.G. et al. (2003) J. Biol. Chem. 278:24200.

11. Lin, C.G. et al. (2003) J. Biol. Chem. 280:8229.

12. Calhabeu, F. et al. (2006) Exp. Cell. Res. 312:1876.

• Long Name:

  Nephroblastoma Overexpressed Gene

• Entrez Gene IDs:

  4856 (Human); 18133 (Mouse)

• Alternate Names:

  CCN family member 3; CCN3; CCN3IGF-binding protein 9; IBP-9; IGFBP-9; IGFBP9novH; Insulin-like growth factor-binding protein 9; nephroblastoma overexpressed gene; Nephroblastoma-overexpressed gene protein homolog; NOV; NOVH; protein NOV homolog