• Species Reactivity

  Human, Mouse

• Specificity

  Detects endogenous human and mouse SALL4 in Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 651839

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human SALL4    
  Lys96-Gly359    
  Accession # Q9UJQ4

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.025 µg/mL

  See below

• 
  

• Immunohistochemistry

  8-25 µg/mL

  Immersion fixed paraffin-embedded sections of human testis

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SALL4

SALL4 (SAL-like protein 4) is a 1324 amino acid (aa) member of the SAL protein family that possess multiple C  ₂H  ₂-type zinc-fingers. It is a transcriptional repressor when associated with histone deacetylase and a transcriptional activator of the Wnt pathway in its native form. It contributes to generation of induced pluripotent stem cells and to axis formation during development. It is frequently overexpressed in germ cell tumors and acute myeloid leukemias. Mutations of human SALL4 can result in conditions such as Okihiro, Duane Radial Ray, or Holt-Oram syndromes. The region used as an immunogen is common to both SALL4 and the ubiquitous 617 aa isoform, SALL4B; it shares 76% aa identity between human and mouse.

• Long Name:

  Sal-like 4

• Entrez Gene IDs:

  57167 (Human); 99377 (Mouse)

• Alternate Names:

  dJ1112F19.1; DRRS; HSAL4; MGC133050; SALL4; sal-like 4 (Drosophila); sal-like protein 4; Zinc finger protein 797; Zinc finger protein SALL4; ZNF797; ZNF797sal (Drosophila)-like 4