• Species Reactivity

  Human

• Specificity

  Detects human CLP24 in ELISAs.

• Source

  Monoclonal Mouse IgG    _2B Clone # 652424

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human CLP24    
  Accession # Q9BSN7

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  2.5 µg/10    ⁶ cells

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLP24

TMEM204, also known as Claudin-like protein of 24 kDa (CLP24), is a cell surface protein with four transmembrane domains and a C-terminal PDZ motif. TMEM204 localizes to adherens junctions between epithelial and endothelial cells. It is involved in regulating paracellular flux and is upregulated under hypoxic conditions. Human TMEM204 shares 88% aa sequence identity with mouse and rat TMEM204.

• Long Name:

  Claudin-like Protein 24

• Entrez Gene IDs:

  79652 (Human); 407831 (Mouse); 287129 (Rat)

• Alternate Names:

  C16orf30; C16orf30chromosome 16 open reading frame 30; Claudin-like protein 24; CLP24; CLP24MGC111564; FLJ20898; TMEM204; transmembrane protein 204