• Species Reactivity

  C. botulinum

• Specificity

  Detects recombinant C. botulinum BoNT-D Heavy Chain in direct ELISAs and Western blots. 100% and approximately 10% cross-reactivity with recombinant C. botulinum BoNT-C1 Heavy Chain is observed in Western blots and direct ELISAs, respectively. In direct ELISAs, no cross-reactivity with the Heavy Chains of BoNT-A, -E, or -G or the Light Chains of BoNT-A, -B, -C1, -D, -E, -F, -G is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 682810

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant Clostridium botulinum Neurotoxin Type D Heavy Chain    
  Asn862-Glu1276    
  Accession # P19321

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant     C. botulinum BoNT-D Heavy Chain    
   

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BoNT-D Heavy Chain

BoNT-D (Botulinum neurotoxin serotype D) is produced by Clostridium botulinum and inhibits acetylcholine release from neuromuscular junctions. Notably, BoNT-D is not toxic to human cells. The 1276 amino acid (aa) BoNT-D precursor is cleaved to generate a disulfide-linked dimer of the 50‑55 kDa light chain peptidase and the 100 kDa heavy chain. The heavy chain contains receptor binding and pore forming domains. The light chain translocates through this pore to the target cell cytosol where it cleaves synaptobrevin, thus blocking synaptic vesicle fusion with the presynaptic membrane. Within aa 862-1276 of the heavy chain, BoNT-D shares 31%, 33%, and 41% aa sequence identity with BoNT-A, -B, and -C1 heavy chains, respectively.

• Long Name:

  Botulinum Neurotoxin Type D Heavy Chain

• Alternate Names:

  BoNTD Heavy Chain; BoNT-D Heavy Chain