• Species Reactivity

  Human

• Specificity

  Detects human Desmoglein-4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Desmoglein-1, -2, or -3 is observed and approximately 40% cross-reactivity with recombinant mouse Desmoglein-4 is observed.

• Source

  Monoclonal Mouse IgG    _2A Clone # 683135

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Desmoglein-4    
  Glu24-Ala631    
  Accession # Q86SJ6

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desmoglein-4

Desmoglein-4 (DSG4) is a 110-130 kDa type I transmembrane glycoprotein in the cadherin family of calcium-dependent cell adhesion molecules. Desmogleins interact with transmembrane Desmocollins to form the adhesive components of desmosomes on epithelial cells. Desmoglein-4 contains four cadherin repeats in its extracellular region and two desmoglein repeat domains in its cytoplasmic region. Alternate splicing generates an additional isoform with a substitution in the cytoplasmic region. Desmoglein-4 is expressed on keratinocytes and trichocytes in the epidermis and hair follicles. It is required for hair follicle development, and DSG4 mutations are associated with autosomal recessive hypotrichosis. Within amino acids 24-631 (which includes the 26 aa propeptide and the ECD), human Desmoglein-4 shares 82% aa sequence identity with mouse and rat Desmoglein-4.

• Entrez Gene IDs:

  147409 (Human); 16769 (Mouse); 291754 (Rat)

• Alternate Names:

  Cad13; Cadherin family member 13; CDGF13; CDHF13; CDHF13CDH family member 13; desmoglein 4; Desmoglein4; Desmoglein-4; DSG4; LAHdesmoglein-4