• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human Tmp21/p23 in direct ELISAs and Western blots.    
   

• Source

  Monoclonal Mouse IgG    _2A Clone # 805125

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E.coli-derived recombinant human Tmp21/p23    
  Ile32-Arg185    
  Accession # P49755

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tmp21/p23

Tmp21 (Transmembrane protein 21 also TMED-10,Transmembrane emp24 Domain-containing Protein 10, p24δ1 and p23) is a 21‑23 kDa member of the EMP [endosome membrane protein] 24 /GP25L family of cargo molecules. It is ubiquitously expressed, being found associated with the ER-Golgi complex. Molecules destined for secretion are folded/processed in the ER, transported out of the ER via COP-II coated vesicles, united with other molecules in similar vesicles to form an ERGIC/ER-Golgi Intermediate Compartment, and finally delivered to the Golgi (or returned to the ER) via new (presumably COP-I) coated vesicles for terminal maturation. Tmp21 is considered part of both the COP-II and COP-I oligomer complex, the latter that arises from the ERGIC and both stabilizes the ER and allows for the selection of specific molecules targeted for secretion. It also is known to associate with the presenilin complex in the plasma membrane and downregulate gamma ‑secretase activity. This impacts A beta production but not AICD generation. Mature human Tmp21 is a 188 amino acid (aa) type I transmembrane protein. It contains a 154 aa luminal region (aa 32-185) that shows a membrane interaction GOLD (Golgi Dynamics) domain (aa 41-193), and a short 13 aa cytoplasmic tail (aa 207-219) that binds to COP vesicle coat complexes. Exists as both a monomer and homodimer. Over aa 32-185, human Tmp21 shares 97% aa sequence identity with mouse Tmp21.

• Long Name:

  21 kDa Transmembrane Trafficking Protein

• Entrez Gene IDs:

  10972 (Human); 68581 (Mouse); 84599 (Rat)

• Alternate Names:

  21 kDa transmembrane-trafficking protein; p23; P24(DELTA); p24delta; S31I125; S31III125; TMED10; Tmp21; TMP21Tmp-21-I; transmembrane emp24 domain-containing protein 10; transmembrane emp24-like trafficking protein 10 (yeast); Transmembrane protein Tmp21,21 kDa transmembrane trafficking protein