• Species Reactivity

  Human

• Specificity

  Detects human MDGA2 in direct ELISAs.    
   

• Source

  Monoclonal Mouse IgG    _2B Clone # 803732

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human MDGA2    
  Gln21-Asp934    
  Accession # Q72553

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MDGA2

MDGA2 (MAM domain-containing glycosylphosphatidylinositol anchor protein 2; also named MAMDC1) is a 130 kDa member of the Ig superfamily of proteins (1). Human MDGA2 is synthesized as a 956 amino acid (aa) precursor that contains a 25 aa signal sequence, a 906 aa mature chain, and a 25 aa propeptide. The mature chain consists of six Ig-like domains, followed by a MAM domain (aa 746‑921) and a GPI anchor. In addition, there are eight potential sites for N-linked glycosylation. Mature human MDGA2 shares 98% aa sequence identity with mature mouse and rat MDGA2. MDGA2 is structurally similar to other IgCAMS, such as the L1 family and axonin 1, which have roles in cell adhesion, migration, and process outgrowth (2). Northern blot analysis shows MDGA2 expression is limited to the central and peripheral nervous system (1). Within the brain, moderate expression is observed in the cerebral cortex, the hindbrain, the basilar pons, the neocortex, the hippocampus, the amygdala, olfactory bulb, and selected nuclei of the thalamus (1). The similarity of MDGA2 to other Ig-containing molecules, and its temporal‑spatial patterns of expression within restricted neuronal populations, suggest a role for MDGA2 in regulating neuronal migration, as well as other aspects of neural development, including axon guidance (1). One study shows that MDGA2 gene is implicated in neuroticism (3).

• References:

1. Litwack, E.D. et al. (2004) Mol. Cell. Neurosci. 25:263.

2. Takeuchi, A. and D.D.M. O'Leary (2006) J. Neurosci. 26:4460.

3. van den Oord, E.J. et al. (2008) Arch Gen Psychiatry 65:1062. 

• Long Name:

  MAM Domain Containing Glycosylphosphatidylinositol Anchor 2

• Entrez Gene IDs:

  161357 (Human); 320772 (Mouse); 314180 (Rat)

• Alternate Names:

  MAM domain containing glycosylphosphatidylinositol anchor 2; MAMDC1; MDGA2