• Species Reactivity

  Human

• Specificity

  Detects human Cytokeratin 19 in direct ELISAs and Western blots.    
   

• Source

  Monoclonal Mouse IgG    _2A Clone # 963420

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E.coli-derived recombinant human Cytokeratin 19    
  Gln311-Lys370    
  Accession # P08727

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.25 µg/mL

  See below

• 
  

• Immunohistochemistry

  5-25 µg/mL

  See below

• 
  

• Immunocytochemistry

  5-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytokeratin 19

Cytokeratin 19 (Keratin, type I cytoskeletal 19; also KRT-19, CK19 and Keratin-19) is a 40-45 kDa, acidic Class I keratin member of the intermediate filament family of proteins. Individual keratins are always expressed in tandem with a second keratin, and these are found in all epithelial cells. The class I KRT-19 heterodimerizes/polymerizes with 50-52 kDa class II KRT-8 (plus KRT-5 and -7) to form 8-10 nm filaments in epidermal stem cells, secretory gland (sweat; mammary; bile duct) simple epithelium, and neuroendocrine epidermal Merkel cells. It may represent a viable marker for skin stem cells. In skin, Cytokeratin 19 forms filaments in the fetal epithelium, and then progressively decreases with age, being virtually absent by age 17. Human Cytokeratin 19 is 400 amino acids (aa) in length. It contains an N-terminal "head" region (aa 1-79) and a subsequent "rod" region (aa 80-387), but is absent a typical C-terminal tail region. Cytokeratin 19 possesses at least 5 utilized phosphorylation sites plus one acetylated Lys residue. Based on other keratins, and the presence of an Asp at position 238, there may be caspase cleavage-generated isoforms. Full length human Cytokeratin 19 (aa 2-400) shares 82% aa sequence identity with mouse Cytokeratin 19.

• Entrez Gene IDs:

  3880 (Human); 16669 (Mouse)

• Alternate Names:

  CK19; CK-19; Cytokeratin 19; Cytokeratin-19,40-kDa keratin intermediate filament; EndoC; K19; K19cytokeratin-19; K1CS; keratin 19; keratin, type I cytoskeletal 19; keratin, type I, 40-kd; keratin-19; Krt19; MGC15366